Luteinizing Hormone (LH) is always present in your urine and increases just before ovulation. This increase or “surge” in LH triggers ovulation, which is the release of an egg from your ovaries and the most fertile time of your cycle. The FIRST RESPONSE® Daily Ovulation Test detects the LH surge, which precedes ovulation by 24-36 hours. Your two most fertile days begin with the LH surge. If you have intercourse within the next 24-36 hours of your surge, you will maximise your chances of becoming pregnant.
The FIRST RESPONSE® Daily Ovulation Test is over 99% accurate in detecting the LH surge in laboratory studies.
Most women can start testing the day after their periods ends. The table below will help you determine when to begin testing.

You may test at any time of the day, but you should test at approximately the same time each day. Reduce your liquid intake for 2 hours before testing.
Remove a test stick from the pouch and IMMEDIATELY RESEAL THE POUCH with the plastic zipper by pressing the two sides together with your fingers to form a seal. IT IS IMPORTANT THE POUCH IS TIGHTLY SEALED. Perform the test immediately after removing the test stick from the pouch.

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Holding the test stick by the Thumb Grip with the Absorbent Tip pointing downward and the Result Window facing away from your body, place the Absorbent Tip in your urine stream for 5 seconds only |
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You may also collect your urine in a clean, dry cup and immerse the entire Absorbent tip in the urine for 5 seconds only. |
READ YOUR RESULT AT 5 MINUTES
READ THE COLOUR & INTENSITY OF THE LINES NOT THE THICKNESS
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LH SURGE. Two lines are visible and the colour and intensity of the Test Line is similar to or darker than the Reference Line. You should ovulate within the next 24-36 hours. You can stop testing for this cycle. |
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No LH Surge. Two lines are visible but the Test Line is lighter than the Reference Line or there is a Reference Line and NO visible Test Line. You should continue with daily testing until the Reference Line and the Test Line have the same colour intensity or the Test Line is darker than the Reference Line. |
There should be at least one pink line in the Result Window at five minutes. If not, the test is invalid. This could be due to insufficient urine on the Absorbent Tip or not laying the test stick on a flat surface with the Result Window facing up. You should retest with another stick, carefully following all directions.
First, it is important to remember that it can take normal, healthy couples many months to become pregnant. There are many factors which affect your ability to become pregnant even if you have been able to have intercourse during your most fertile time. If after several months, you have no success, consult your doctor. Since you have been monitoring your LH surge with the FIRST RESPONSE® Daily Ovulation Test, you will be able to give your doctor a clearer picture of how you have been ovulating.
You can visit our “Trying for a baby?” page to Try our simple-to-use food wheels and discover the exact foods and portions you can choose to include in your day-to-day diet to optimise your health and fertility.
To increase your chances of a healthy pregnancy for you and your baby, you should consider the following:
Certain medical conditions may adversely affect the reliability of this test for predicting ovulation. These include pregnancy, postpartum, post-abortion, polycystic ovary syndrome (PCOS), ovarian cysts, the onset of menopause, and untreated hypothyroidism. On prescription drugs, such as menotropins for injection (Pergonal1) and danazol (Danocrine2), may affect the result you see. If you are undergoing therapy with Clomiphene citrate (e.g., Clomid3 or Serophene4), please consult your doctor for the appropriate time to begin testing. Medications containing hCG or LH may affect the test and should not be taken whilst using this test. Women with medically diagnosed fertility problems should consult their doctor before using this product.
1 Pergonal is a registered trademark of Ares Trading SA.
2 Danocrine is a registered trademark of Sanofi-Synthelabo, Inc.
3 Clomid is a registered trademark of Merrell Pharmaceutical.
4 Serophene is a registered trademark of Serono Laboratories, Inc.
After using the pill your cycle may be irregular and may take some time to stabilise again. You may wish to wait until you have had two normal periods before starting to use the FIRST RESPONSE® Daily Ovulation Test. Follow your doctor’s advice when to best try to become pregnant.
Sperm can survive up to 72 hours (3 days), and this test predicts your LH surge only up to 24-36 hours in advance. Therefore, if you have intercourse before you discover your surge, the egg can still be fertilised
For further information, or advice on using the FIRST RESPONSE® Daily Ovulation Test, contact the FIRST RESPONSE Advice Line at Church & Dwight UK Ltd between 9.00am and 4.00pm Monday - Friday. Ask for Carol Moore on 01303 221686. From outside the UK call +44 1303 221 906. In correspondence, please quote the lot number printed on the carton.